Specifications
1 Microscope
• Inverted stand: Axio Observer
• Upright stand: Axio Examiner, Axio Imager
• Port for coupling of ELYRA
• Camera port
• Manual or motorized stages
• Incubation solutions
• Fast Z piezo inserts
• Definite Focus
2 Objectives
• C-APOCHROMAT
• Plan-APOCHROMAT
• W Plan-APOCHROMAT, Clr Plan-APOCHROMAT, Clr Plan-NEOFLUAR
• LCI Plan-APOCHROMAT
3 Illumination
• UV laser: 355 nm, 405 nm
• VIS laser: 440 nm, 458 nm, 488 nm, 514 nm, 543 nm, 561 nm, 594 nm, 633 nm
• NIR laser for multiphoton imaging: Ti:Sa, OPO*, InSight DeepSee*, Discovery*
4 Detection
• 3 or 34 descanned spectral channels
(GaAsP and/or multialkali PMT)
• Airyscan detector with optional Fast module
• 2 additional GaAsP channels (BiG.2)
• Up to 6 non-descanned GaAsP detectors
• Up to 12 non-descanned GaAsP or PMT detectors total
• Transmitted light detector (T-PMT)
5 Software
• ZEN, recommended modules:
Tiles & Positions, Experiment Designer, FRAP, FRET, RICS, FCS, Deconvolution, 3Dxl Viewer – powered by arivis
Scanning Module
Scanner Two independent, galvanometric scanning mirrors with ultrashort line and frame flyback
Scanning Resolution 4 × 1 to 8192 × 8192 pixels, also for multiple channels, continuously adjustable
Scanning Speed 19 × 2 speed levels; up to 13 images/sec. with 512 × 512 pixels (max. 430 images/sec. 512 × 16), up to 6875 lines/sec.
In Fast Airyscan mode: 13×2 speed levels, up to 19 images/sec. with 512×512 (max. 27 images/sec. 480×480, or 6 images/sec. 1024×1024)
Scanning Zoom 0.6 × to 40 ×; digitally adjustable in increments of 0.1 (Axio Examiner: 0.67 × to 40 ×)
Scanning Rotation Can be rotated freely (360 degrees), adjustable in increments of one degree, freely adjustable XY offset
Scanning Field 20 mm field diagonal (max. 18 mm for Axio Examiner) in the intermediate image plane, with full pupil illumination
Pinholes Master pinhole with preset size and position; can be adjusted as desired for multitracking and short wavelengths (such as 405 nm)
Beam Path Exchangeable Twin Gate beam splitter with up to 100 combinations of excitation wavelengths and outstanding laser line suppression;
manual interface port for external detection modules (such as BiG.2, Airyscan, third party detectors, internal detection
with spectral signal separation and signal recycling loop for compensation of polarization effects)
Detection Options
Detectors 3 or 34 spectral detection channels, GaAsP and /or multialkali PMT (QE 45% typical for GaAsP)
2 additional GaAsP detection channels (BiG.2)
Airyscan detector (32 channels GaAsP), delivers resolution up to 140 nm lateral, 400 nm axial; in Fast mode: 145/180 nm lateral, 450 nm axial
Up to 12 non-descanned detection channels (PMT and/or GaAsP)
Transmitted light detector (PMT)
Spectral Detection 3 or 34 simultaneous, confocal reflected-light channels, GaAsP and /or PMT based
freely adjustable spectral detection area (resolution down to 3 nm)
Data Depth 8 bit, 12 bit or 16 bit available; up to 35 channels simultaneously detectable
Real-Time Electronics Microscope, laser, scanning module and additional accessory control; data acquisition and synchronization management through real-time
electronics; oversampling read-out logic; ability to evaluate data online during image acquisition
ZEN Imaging Software
System Configurations Workspace to conveniently configure all of the motorized functions of the scanning module, laser and microscope; save and restore application configurations (Re-use)
System Self Rest Calibration and testing tool to automatically test and calibrate the system
Recording Modes, Smart Setup: Spot, Line/Spline, Frame, Tiles, Z Stack, Lambda Stack, Time Series and all combinations (XYZ, lambda, t), online calculation and visualization of ratio images, average and summation (by line/image, adjustable), Step Scan (for higher image frame rates);
quick set up of imaging conditions using Smart Setup by simply selecting the labelling dye
Crop Function Easily select scanning areas (simultaneously select zoom, offset, rotation)
Real ROI Scan, Spline Scan: Scans of up to 99 designated ROIs (regions of interest) as desired and pixel-by-pixel laser blanking; scan along a freely defined line
ROI Bleaching Localized bleaching in up to 99 bleach ROIs for applications such as FRAP (fluorescence recovery after photobleaching) or uncaging; use of different speeds for bleaching and imaging, use of different laser lines for different ROIs
Multitracking Rapidly change excitation lines when recording multiple fluorescences for the purpose of minimizing signal crosstalk and increasing dynamic range
Fast Acquisition Fast mode scan with 4x parallelisation in Y-direction, detection by Airyscan module
Lambda Scan Parallel or sequential acquisition of image stacks with spectral information for every pixel
Linear Unmixing Acquisition of crosstalk-free, multiple fluorescence images using simultaneous excitation; online or offline and automatic or interactive unmixing; advanced unmixing logic with indication of reliability
Visualization XY, orthogonal (XY, XZ, YZ), Cut (3D section); 2.5D for time series of line scans, projections (maximum intensity); animations;
Depth coding (inverse colors), brightness, gamma and contrast settings; color table selection and modification (LUT), character functions
Image Analysis and Operations : Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more; operations: addition, subtraction, multiplication, division, ratio, shift, filters (low-pass, median, high-pass, etc., also user-definable)
Image Management Features for managing images and the corresponding imaging parameters; multiprint feature; streaming of acquisition data for online processing of large data sets
Applications
Live cell imaging, FRET, FRAP, Co-localisation analysis, Spectral Mixing, 3D Recontruction