Model – YEA955 (Waters)
High-resolution MS (HRMS) is rapidly advancing into many fields of modern analytical sciences. Instruments such as Fourier-transform ion cyclotron resonance (FTICR) and modern orbitrap and TOF systems are now frequently used in laboratories, where only a few years ago traditional quadrupole and ion trap mass spectrometers dominated. In particular, the selective data-acquisition modes of triple quadrupole mass spectrometers (e.g., precursor ion scan or multiple reaction monitoring) are increasingly being replaced by full-scan or MS/MS experiments on HRMS instruments even in quantitative applications, because the information gained from high-resolution, accurate mass data often outweighs the benefits of highly selective measurements on low-resolution mass spectrometers.
Principle:-
A mass spectrometer generates multiple ions from the sample under investigation, it then separates them according to their specific mass-to-charge ratio (m/z), and then records the relative abundance of each ion type.
The first step in the mass spectrometric analysis of compounds is the production of gas phase ions of the compound, basically by electron ionization. This molecular ion undergoes fragmentation. Each primary product ion derived from the molecular ion, in turn, undergoes fragmentation, and so on. The ions are separated in the mass spectrometer according to their mass-to-charge ratio, and are detected in proportion to their abundance. A mass spectrum of the molecule is thus produced. It displays the result in the form of a plot of ion abundance versus mass-to-charge ratio. Ions provide information concerning the nature and the structure of their precursor molecule. In the spectrum of a pure compound, the molecular ion, if present, appears at the highest value of m/z (followed by ions containing heavier isotopes) and gives the molecular mass of the compound.
Usage:
1Chemistry and Biochemistry
2Pharmaceutical and Drug Development
3Forensic Science
4Environmental Analysis