Biomedical-Photonics Lab

Publications

 

  • “Unravelling changes in single-cell osteoarthritic chondrocytes through coupling of Raman spectroscopy and multivariate curve resolution-alternating least square (MCR-ALS) algorithm.” Gavish Uppal, Tarun Goyal, Anup Kumar and Rajesh Kumar. Microchemical Journal, Volume 212, 113264, 2025, https://doi.org/10.1016/j.microc.2025.113264.

 

  • “First-order Analysis of Stromal Collagen in Chronic Pancreatitis Using Cross-modality Image Translation with Convolutional Neural Networks”. Nisha, Garima Singhal, Gavish Uppal and Rajesh Kumar. Procedia Computer Science, Elsevier, Volume 258 (pages 365-373),2025,  https://doi.org/10.1016/j.procs.2025.04.273

 

  • “Machine learning model to study single cell osteoarthritic chondrocytes via Raman spectroscopy”. Gavish Uppal, Nisha, Tarun Goyal, Anup Kumar, Rajesh Kumar. Lecture Notes in Networks and Systems-2025, ISSN:2367-3389, Springer (accepted).

 

  • “Assessment of Collagen Morphology in Pancreatic Tumor using Computational Translation of Histological Images”. Garima Singhal, Nisha, Gavish Uppal, Manjit Kaur, Ruchi Sinha and Rajesh Kumar. Lecture Notes in Networks and Systems-2025, (Best Paper Award at International Conference AICTA-2024), ISSN:2367-3389, Springer (accepted).

 

  • “Virtual Collagen Imaging Translated from Histopathological Whole-core Microscopy Images for Quantitative Analysis of Collagen Remodeling in Pancreatic Lesions”  Varun Nair, Gavish Uppal, Sukrit Gupta, Ruchi Sinha, Manjit Kaur and Rajesh Kumar, 2025 (submitted)

 

  • “Laser Technologies: Cutting-Edge Applications in Biomedical Science”. Sreelakshmi Sreeraj, Achsah Thankam John, Anusha Thomas, Rajesh Kumar, Guan-Yu Zhuo, Yury Kistenev, Subir Das, Ajeetkumar Patil, Nirmal Mazumder, Journal of Biophotonics, 2025 (submitted)

 

  • “Two-photon microscopy.” Kausalya Neelavara Makkithaya, Gagan Raju, Subir Das, Ajeetkumar Patil, Rajesh Kumar, Nisha, Yury V. Kistenev, Vishwachetan S Arakeri, Guan Yu Zhuo, Nirmal Mazumder. Comprehensive Biomedical Physics (2nd edition), Elsevier, 2025 (accepted).

 

  • “Development of a Portable, Wireless, Multi-channel Physiograph with Integrated Stimulator”.  R S Halder, B. Basumatary, R Kumar, A K Sahani. IEEE Transactions on Biomedical Engineering, 2025 (submitted)

 

  • “Comparative Analysis of Constitutive Viscoelastic Models Under Unconfined Compression of Osteoarthritic Femoral Articular Cartilage: An Ex-Vivo Study.” Gavish Uppal, Jaganath Anand Gaonkar, Tarun Kumar, Anup Kumar and Rajesh Kumar. 2025, Cell Press (submitted).

 

  • “Smart and Sustainable Nanomaterials for Digital Healthcare: Application in Drug Delivery.” Richa Pal, Rajesh Kumar, Trishant Kumar, Danveer Singh, Kishant Kumar. Book Chapter, Springer Nature 2025 (Submitted).

 

  • “Linking Macroscopic and Microscopic Grading Scores of Osteoarthritic Human Articular Cartilage: An Association Between the ICRS and OARSI Scoring Systems.” Gavish Uppal, Tarun Goyal, Anup Kumar, Ruchi Sinha, Manjit Kaur and Rajesh Kumar, 2025 (submitted).

 

  • “AI-Enhanced FES for Foot Drop: A Novel Biphasic Charge-Balanced Stimulation Approach.”  S. Halder, A N Mallick, A Khokhar, R. Bansal, R. Kumar, A. K. Sahani. IEEE Transactions on Biomedical Engineering, 2025 (TBME-Submitted).

 

  • “A Systematic Review on Wireless Biopotential Signal Acquisition Devices.” R S Halder, B. Basumatary, R Kumar, A K Sahani. IETE Technical Review, 2025 (Submitted).

 

  • “Optical Screening of Citrus Leaf Diseases Using Label-Free Spectroscopic Tools: A Review”. Saurav Bharadwaj, Akshita Midha, Shikha Sharma, Gurupkar Singh Sidhu, Rajesh Kumar. Journal of Agriculture and Food Research (2024), 101303, ISSN: 2666-1543, https://doi.org/10.1016/j.jafr.2024.101303.

 

  • “Quantitative analysis of collagen remodeling in pancreatic lesions using computationally translated collagen images derived from brightfield microscopy images”.Varun Nair, Gavish Uppal, Saurav Bharadwaj, Ruchi Sinha, Manjit Kaur and Rajesh Kumar. arXiv:2304.12725 (2023). https://doi.org/10.48550/arXiv.2304.12725

 

  • “Viscoelastic properties of osteoarthritic human knee articular cartilage”. Jaganath Anand Gaonkar and Rajesh Kumar. 2022 IEEE 4th Eurasia Conference on Biomedical Engineering, Healthcare and Sustainability (ECBIOS), 2022, pp. 83-87, ISBN:978-1-7281-9579-7, doi: 10.1109/ECBIOS54627.2022.9944993

 

  • “The assessment of reflectance ratios for determination of water content in leaves using shorter end of near-infrared spectroscopy”. Saurav Bharadwaj and Rajesh Kumar. SPIE Proceedings Volume 12144, Biomedical Spectroscopy, Microscopy, and Imaging II; 121440O (2022). doi.org/10.1117/12.2624474

 

  • “Assessment of Articular Cartilage by Second Harmonic Microscopy: Challenges and Opportunities”. Rajesh Kumar and Anup Kumar. Frontiers in Physics. Medical Physics and Imaging 2019, 7(137); doi: 10.3389/fphy.2019.00137

 

  • “Label-Free Non-linear Multimodal Optical Microscopy – Basics, Development, and Applications.” Nirmal Mazumder, Naveen K. Balla, Guan-Yu Zhuo, Yury V. Kistenev, Rajesh Kumar, Fu-Jen Kao, Sophie Brasselet, Viktor Nikolaev, Natalya Krivova, Frontiers in Physics. Optics and Photonics. 2019 7(170); doi: 10.3389/fphy.2019.00170

 

  • “Comparison of Compressive Stress-Relaxation Behavior in Osteoarthritic (ICRS Graded) Human Articular Cartilage”. Rajesh Kumar, David M. Pierce, Vidar Isaksen, Catharina de Lange Davies, Jon O. Drogset, Magnus B. Lilledahl. International Journal of Molecular Sciences (MDPI) 2018, 19(2), 413; doi:10.3390/ijms19020413.

 

  • “Automated calibration and control for polarization resolved second harmonic generation on commercial microscopes”.Elisabeth I. Romijn, Andreas Finnøy, Rajesh Kumar and Magnus B. Lilledahl. PLOS ONE (2018); doi:10.1371/journal.pone.0195027

 

  • “Multiphoton microscopy of osteoarthritic articular cartilage”.Rajesh Kumar, Catharina L. Davies, Jon O. Drogset and Magnus B. Lilledahl. Optics in the Life Sciences, OSA Technical Digest ISBN: 978-1-943580-25-5, Optical Society of America (2017), https://doi.org/10.1364/NTM.2017.NW4C.4

 

  • “Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage.”Rajesh Kumar, Kirsten M. Grønhaug, Catharina L. Davies, Jon O. Drogset, and Magnus B. Lilledahl. Biomedical Optics Express 2015, 6(5), 1895-1903. DOI: 10.1364/BOE.6.001895

 

  • “Polarization second harmonic generation microscopy provides quantitative enhanced molecular specificity for tissue diagnostics”.Rajesh Kumar, Kirsten M. Grønhaug, Elisabeth I. Romijn, Andreas Finnøy, Catharina L. Davies, Jon O. Drogset and Magnus B. Lilledahl. Journal of Biophotonics, Wiley-VCH, 8(9):730-9 (2015), DOI: 10.1002/jbio.201400086

 

  • “High resolution pictures reveal signs of osteoarthritis. Høyoppløste bilder avslører tegn på slitasjegikt”. Rajesh Kumar. Popular Science Article, published in forskning.no(2015)

 

  • “Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study” . Rajesh Kumar, Gajendra P. Singh, Kirsten M. Grønhaug, Nils Afseth, Catharina L. Davies, Jon O. Drogset and Magnus B. Lilledahl. International Journal of Molecular Sciences 2015, 16(5), 9341-9353; DOI:10.3390/ijms16059341

 

  • “Optical investigation of osteoarthritic human cartilage (ICRS Grade) by confocal Raman spectroscopy: A pilot study”. Rajesh Kumar, Kirsten M. Grønhaug, Nils K. Afseth, Vidar Isaksen, Catharina de Lange Davies, Jon O. Drogset and Magnus B. Lilledahl, Analytical and Bioanalytical Chemistry (Springer)), 407(26):8067-77 (2015). DOI: 10.1007/s00216-015-8979-5

 

  • “Mueller matrix three-dimensional directional imaging of collagen fibers”.Pål G Ellingsen, Lars M Sandvik Aas, Vegard S Hagen, Rajesh Kumar, Magnus B Lilledahl, Morten Kildemo. Journal of Biomedical Optics 19(2), 026002 (2014).

 

  • “Analysis of human knee osteoarthritic cartilage using polarization sensitive second harmonic generation microscopy”.Rajesh Kumar, Kirsten M. Grønhaug, Elisabeth I. Romijn,Jon O. Drogset‎,Magnus B. Lilledahl. of SPIE- the international society for optical engineering. Vol. 9129, 91292Z (2014). DOI: 10.1117/12.2051989.

 

  • “A facile and real-time spectroscopic method for biofluid analysis in point-of-care diagnostics”. Rajesh Kumar, Singh GP, Barman I, Dingari NC, Nabi G. Bioanalysis, Journal of Future Science Group (fsg). 5(15):1853-61 (2013). DOI: 10.4155/bio.13.126.

 

  • “Selective sampling using confocal Raman spectroscopy provides enhanced specificity for urinary bladder cancer diagnosis”. Barman I, Dingari NC, Singh GP, Rajesh Kumar, Lang S, Nabi G. Journal of Analytical and Bioanalytical Chemistry. 2012; 404(10):3091-9. DOI: 10.1007/s00216-012-6424-6.

 

  • “Fabrication and fluorescence characterization of dye doped graded index polymer optical fibre preform”. Kailasnath, T.S. Sreejaya, Rajesh Kumar, P.Radhakrishnan, VPN.Nampoori and CPG. VaIlabhan. Journal of Optics and Laser Technology 40,687,2008. DOI: 10.1016/j.optlastec.2007.10.015.

 

  • “Optical Screening of Citrus Leaf Diseases Using Label-Free Spectroscopic Tools: A Review“. Saurav Bharadwaj, Akshita Midha, Shikha Sharma, Gurupkar Singh Sidhu, Rajesh Kumar. arXiv (2024), arXiv:2403.04820.https://doi.org/10.48550/arXiv.2403.04820

 

 

Others

 

  • “Photonics in Plant Pathology: Precision Techniques for Disease Detection. .” Nisha and Rajesh Kumar, National Symposium of Himalayan Phytopathological Society on New Vistas in Plant Pathology Research, November 7-8, 2024, CSKHPKV, Palampur, Himachal Pradesh.

 

  • “Single Cell, Label Free analysis of Chondrocytes Nucleus during progression of Osteoarthritis by Raman Spectroscopy.” Gavish Uppal, Tarun Goyal and Rajesh Kumar. International Research Fair Anusandhan 2.0, IIT Mandi, India, June 18-19, 2024.

 

  • “Automated Polarization Second Harmonic Generation”. Elisabeth I. Romijn, Andreas Finnøy, Rajesh Kumar, and Magnus B. Lilledahl. SCANDEM, Nordic Microscopy Society (2016), Trondheim, Norway.

 

  • “Mueller Matrix imaging on biomedical samples – collagen fibers and atherosclerotic plaques”. Pål Gunnar Ellingsen, Lars Martin Sandvik Aas, Vegard Hagen, Rajesh Kumar, Håkan Gustafsson, Magnus Borstad Lilledahl, Morten Kildemo, Mikael Lindgren. 6th International Conference on Spectroscopic Ellipsometry, May 26-31, 2013 Kyoto, Japan.

 

  • “Multi-Photon Microscopy: Adding prospective new dimensions to Osteoarthritis”.Rajesh Kumar and Magnus Borstad Lilledahl. The 4th National PhD Conference in Medical Imaging, 28 – 29 Nov 2012,Trondheim, Norway.

 

  • “Characterization of collagen fiber in cartilage tissue by Müller matrix and non-linear optical imaging”.Rajesh Kumar, Pål Gunnar Ellingsen, Morten Kildemo and Magnus B. Lilledahl. 5th Annual Norwegian Molecular Imaging Consortium(NorMIC), 25-26 October 2012, University of Bergen, Norway.

 

  • “Intra-operative photonic diagnosis”.Rajesh Kumar, Giardini. M, Melzer. A. and Dholakia. K. 23rd Conference of the Society for Medical Innovation and Technology (SMIT-2011), Israel.

 

  • “Bio-photonic techniques: Physical principles, current and potential applications in radiology”. Rajesh, Giardini. M, Samuel. I, Melzer. A. and Dholakia. K. RSNA- 2010, Radiological Society of North America, Chicago, USA.

 

  • “Integration of Bio-photonic techniques with imaging modalities: Potential application in Medical Science”.Rajesh Kumar, Giardini. M, Melzer. A. and Dholakia. K. Euroscience Open Forum(ESOF) International conference, 3 July 2010, Italy.

 

  • “Fluorescence characterization of dye doped polymer optical fibre preform.” Kailasnath, Rajesh Kumar, PR John, P. Radhakrishnan, VPN,Nampoori and CPG .Vallabhan. International Conerence on Optical materials and Thin films advanced Technology, (OMTAT-2005), October 24-27, 2005, Kochi, India.

 

  • “Fabrication and fluorescence characterization of Rhodamine B doped graded index polymer optical fibre preform.”  Kailasnath, Rajesh Kumar, PR John, P. Radhakrishnan, VPN. Nampoori and CPG .Vallabhan. International Conference on Optics and Lasers (ICOL-200S), December 12-15, 2005, Dehradun, India.

 

  • “An LED based evanescent-wave fiber optic sensor for the tracedetection of Fe2+ ions in water”.Kailasnath, Rajesh Kumar, P. Radhakrishnan, VPN. Nampoori a nd CPG. Vallabhan. National conference on Optics December, 21-22, 2006, IIT Chennai.

 

  • “Fabrication of a compact polymer optical fibre drawing machine”. M. Kailasnath, Rajesh Kumar, P. Radhakrishnan, VPN. Nampoori, Notional Laser symposium. December, 17-20, 2007, M.S. University, Vadodara.

 

 

Invited Talk

 

  • Dr. Rajesh Kumar was invited as lead speaker in at IPS North Zone Meet and National Symposium of Himalayan Phytopathological Society on New Vistas in Plant Pathology Research, November 7-8, 2024, CSKHPKV, Palampur, Himachal Pradesh.

 

  • Dr. Rajesh Kumar was invited to give a talk on Agri-photonics on 15/01/2024 at Massachusetts Institute of Technology-MIT, Cambridge, USA.

 

  • Dr. Rajesh Kumar was invited as lead speaker in Photonics and Device Workshop on 09/07/2024 at Indian Institutes of Science Education and Research (IISER) Mohali.

 

  • Biophotonics Tools for Tissue Imaging and Diagnostics. Photonics and Lasers-Academia Industry Meet (PLAIM), Dec 7th -9th, 2022, IIT Bombay Chapter, Bombay Exhibition Center, Mumbai.

 

  • Faculty Development Programme,Workshop in BIOPHOTONICS funded by SERB-DST, Gov of India. 6-7th March 2021, Institute of Engineering & Technology, Devi Ahilya University, Indore.

 

  • Photonics for Healthcare. Rajesh Kumar, The nanoGUNE Cooperative Research Center (CIC), November 23, 2017, Basque, Spain

 

  • Opportunity for Biophotonics in Future Healthcare (id: URS264981). Rajesh Kumar, NASA-Jet Propulsion Laboratory, California Institute of Technology, May 4, 2017, California, USA

 

  • Advanced Optical Tools and Techniques for Orthopaedics. Rajesh Kumar, Cedars-Sinai Hospital (R&D), May 1, 2017, Los-Angeles, USA

 

  • Advanced Microscopic Analysis of Human Articular Cartilage. Rajesh Kumar, All India Institute of Medical Science (AIIMS)-Patna, Department of Orthopaedics, June 10, 2016, India

 

  • Raman and Fluorescence spectroscopy – applications in life sciences. Rajesh Kumar, Nofima – The Food Research Laboratory, Ås, September 23, 2015, Norway